Figure 1.

Rouleaux formation within the renal microvasculature after renal I/R. (A) Multiphoton imaging of renal microcirculation was conducted using 150 kD FITC dextran 24 hours after renal I/R injury; vascular congestion and stacked red blood cells are evident in the cortical microvasculature after I/R (white arrows). (B) Electron microscopy of the highlighted region in (A) after correlative tissue sampling and subsequent cryofixation after high-pressure freezing demonstrating red blood cell congestion in peritubular capillaries (arrows). (C) Capillary cross section in a control, nonischemic kidney. Sample was cryofixed after high-pressure freezing. Magnifications are listed below electron micrographs. DT, distal tubule; PT, proximal tubule.