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. 2017 Jan 25;28(7):2081–2092. doi: 10.1681/ASN.2016040404

Figure 3.

Figure 3.

HIFD immediately improves vascular congestion in a post-AKI kidney. Live multiphoton imaging of sham kidney (A) or kidneys 24 hours post I/R, immediately before (B) and 30 minutes post–HIFD injection (C) are shown. (D) A line scan was generated and is indicative of average RBC velocity in the capillary space pre– and post–HIFD injection. (E) Average nuclear fluorescent intensity of Hoechst 33342 signal in tubular epithelial cells pre- and post-HIFD; for (D) and (E), data are mean±SEM of fluorescent intensity (arbitrary units) of 10 ROI of images from three separate experiments for sham or pre- and post-HIFD. (F) Fluorescent intensity per unit time in sham versus pre– and post–HIFD-treated rats. Fluorescent intensity (arbitrary units) of the interstitial space fluorescence as a percentage of tubule fluorescence of 10 ROI of images from three separate experiments. *,$P<0.05 for sham versus *pre-HIFD or versus $post-HIFD using paired t test; #P<0.05 for pre-HIFD versus post-HIFD using paired t test.