Figure 2.

Pals1 is essential for the nephron function. (A) Bright-field microscopy of kidney sections from Pals wt/flox-Six2Cre+ (Six2-Cre+) and control mice (Six2-Cre−) shows that Six2-Cre+ kidneys are characterized by cyst-filled and bloodless tissues. (B–D) Periodic acid–Schiff staining of Six2-Cre+ and Six2-Cre− kidney sections shows the formation of multiple cysts in Six2-Cre+ kidneys. (C) Littermate control, higher magnification. (D) Pals1-depleted Six2-Cre+ kidney sections, higher magnification. Numbers mark different cysts, details on the right (d1–4). Scale bar, 50 µm. (E) Distribution of cyst numbers (left) and sizes (right) in Six2-Cre− and Six2-Cre+ kidneys. (F) SDS-PAGE analysis of urine from Six2 Cre− and Six2 Cre+ mice reveals proteinuria in Pals1-depleted mice.