Figure 4.

Reduced Pals1 expression results in altered Hippo signaling. (A) Western blot analysis revealed that Pals1, Taz, and Yap, but not actin (loading control), are downregulated in Pals1-depleted (Six2-Cre+) mice (n=3). (B) Real-time PCR analysis showed increased expression of the Hippo target genes Ctgf, Birc2, Birc5, and Cyr61 in Six2-Cre+ mice (data are shown as mean±SD of at least three independent experiments). (C–D) Colocalization of Yap or Taz with the DNA-specific dye DAPI confirmed the nuclear accumulation of Yap and Taz in Six2-Cre+ cyst-lining cells. Asterisks mark cysts and arrows cyst-lining cells. All data shown in (A–D) were obtained from 4-week-old mice.