Figure 7.

Disassembly of cell-cell contacts inactivates Hippo signaling. (A) Disassembly of cell-cell contacts in MDCK cells (wildtype) cultivated with Ca²⁺ (upper panel) and without Ca²⁺ (lower panel) stained for E-Cadherin (adherens junctions), ZO-1 (tight junctions), and actin cytoskeleton (Phalloidin). Scale bar, 20 µm. (B) Disassembly of cell-cell contacts by Ca²⁺ depletion in confluent wildtype MDCK cells increased nuclear localization of Taz (green) and Yap (red). Scale bars, 20 µm. (C) Ca²⁺ depletion resulted in an increased expression of Yap/Taz target genes CYR61 and BIRC2.