Figure 7.

IL-36 signaling activated NLRP3 inflammasome activation in BMDCs. (A) ELISA analysis of IL-1β protein levels in supernatant of BMDCs from WT, IL-36R KO, and NLRP3 KO mice incubated with or without rIL-36α (150 ng/ml) for 24 hours. (B) Representative Western blots and (C) semiquantitation for NLRP3, pro–IL-1β, and caspase-1 p10 in lysates of BMDCs from WT, IL-36R KO, and NLRP3 KO mice incubated with or without rIL-36α (150 ng/ml) for 24 hours. Data are means±SEM of three replicative results obtained from the experiments. *P<0.01; ***P<0.01; ^#not detectable.