Figure 9.
IL-36 facilitated Th17 differentiation in UUO mice and in vitro T cell activation. (A) Real-time PCR showing relative renal mRNA levels of IL-23p19 and IL-17A in sham control and day14 UUO mice. Data are means±SEM of nine mice per group. (B) BMDCs were incubated with or without rIL-36α (150 ng/ml) for 16 hours, pulsed with OVA323–339 peptides, and then, cocultured with OT-II CD4 T cells. After 4 days, supernatants were collected, and the production of IL-17A was detected by ELISA. Data are means±SEM of three replicative results obtained from the experiments. (C) Real-time PCR showing mRNA levels of specific transcription factors for different Th cells in OT-II CD4 cocultured with BMDCs (preincubated with or without rIL-36α for 16 hours and then, pulsed with OVA323–339 peptides) for 4 days. Data are means±SEM of three replicative results obtained from the experiments. *P<0.05; **P<0.01; ***P<0.01.