Figure 6.

Rtn1a knockdown in renal tubules attenuated early renal injury and renal fibrosis in mice with FAN. Pax8;Rtn1a^RNAi (Rtn1a^RNAi) and Pax8;Luci ^RNAi (Luci ^RNAi) mice received either FA or vehicle injection after 3 weeks’ Dox feeding and Dox was withdrawn at day 7 after FA injection. WT mice injected with FA were treated with TUDCA or vehicle starting at day 1 after FA injection and ending at day 7. Mice were euthanized at either day 3 postinjection for assessing AKI or week 4 postinjection for analysis of renal fibrosis (n=5 in each group). (A) Representative images of H&E-stained kidney sections of mice at 3 days postinjection. Original magnification, ×400. (B) Tubular injury scoring of kidneys at 3 days postinjection in FA-treated mice. (C) Representative images of picrosirius red–stained kidney sections of mice at 4 weeks postinjection. Original magnification, ×400. (D) Picrosirius red–positive areas were quantified as the percentage of the sirius red–positive area to total kidney area per field (n=5 mice). (E) Renal function was assessed at 3 days and 4 weeks postinjection by measuring sCr levels. **P<0.01 and ***P<0.001 compared with vehicle-treated control mice; ^###P<0.001 compared with FA-treated Luci^RNAi or FA-treated WT mice; and ^§§§P<0.001 compared with vehicle-treated, FA-injected WT mice (n=5 in each group).