Figure 8.
RTN1A overexpression in renal tubules exacerbated early renal injury and renal fibrosis in mice with FAN. RTN1A overexpression was induced transiently in renal tubules in Pax8; Rtn1aOE mice by Dox feeding starting 3 weeks before FA injection and ending 7 days after FA injection. Mice were euthanized at either 3 days or 4 weeks after FA injection. (A) Representative images of H&E-stained kidney sections of mice at 3 days postinjection. Original magnification, ×400. (B) Tubular injury scoring of kidneys at 3 days postinjection in FA-treated mice. (C) Representative images of picrosirius red–stained kidney sections of mice at 4 weeks postinjection. Original magnification, ×400. (D) Picrosirius red–positive areas were quantified as the percentage of the sirius red–positive area to total kidney area per field (n=5 mice). (E) Renal function was assessed at 3 days and 4 weeks postinjection by measuring sCr levels. ***P<0.001 compared with vehicle-treated control mice; ###P<0.001 compared with FA-treated WT mice (n=5 in each group).