Figure 4.

Urine albumin excretion and glomerular injury persist in APA-KO mice injected with NTS. (A) Persistence of albuminuria was observed in APA-KO mice 96 hours after injection of NTS but not in wild-type (wt) mice as shown by albumin gel electrophoresis and UACR by ELISA obtained 24 and 96 hours after NTS injections. (B) Histologic examination shows exacerbated renal parenchymal injury in APA-KO mice injected with NTS. Representative light microscopy images of kidney sections stained with periodic acid–Schiff. Tissue was harvested from wt or APA-KO mice 96 hours or 12 days after treatment with NTS injection. At 96 hours, mesangial hypercellularity was observed in both groups. However, only APA-KO mouse glomeruli showed hyalinosis, tuft adhesion to the Bowman’s capsule, and microaneurysms. Tubular atrophy with protein lakes was also seen in APA-KO mice. At 12 days, in addition to the aforementioned changes, parietal cell hyperplasia was noted in APA-KO mice. Scale bars, 200 μm in black and green; 500 μm in yellow; 2 mm in white. *P<0.001; ^#P<0.001.