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(A) Incubation time of 60 min. (B) Incubation time of 120 min. Lane M, molecular weight marker (100 bp Plus Blue DNA Ladder); lane Amp, Amphimerus sp. DNA (1 ng); lane N, negative controls (ultrapure water and no DNA template); and numbers 5–104, analyzed human stool samples. The highlighted red numbers correspond to samples that were positive by one or more applied parasitological methods.
