Table 1. CHIKV isolation from tissue homogenates.

mAb	Dose (mg/kg)	Animal	Tissue Virus Isolation
SVIR002 (anti-lysozyme control)	15	31559	Spleen, Finger Joints
		31055	Finger Joints
		31289	Wrist Joints
		31333	Elbow & Wrist Joints, Hamstring
SVIR001 (anti-CHIKV)	15	31078	Negative
		31296	Negative
		31312	Negative
		31302	Negative
	5	31088	Negative
		31335	Negative
		30430	Negative
		31651	Negative

Three groups of four animals each were treated with control antibody SVIR002 at 15 mg/kg, SVIR001 at 15 mg/kg, or SVIR001 at 5 mg/kg. To isolate CHIKV from the infected joint and muscle tissues an aliquot of tissue homogenate was incubated in C6/36 cells for four days. Following incubation, infectious virus was recovered by plaque assay on Vero cells. Only the virus isolation-positive tissues are listed. The limit of detection was 1 PFU per ml of C6/36 supernatant.