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. 2017 Jun 28;10(4):621–631. doi: 10.1016/j.tranon.2017.05.005

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

A: Glutathione (GSH) levels in OvCa cells. GSH is one of the major antioxidants. Among OvCa cell lines, intracellular GSH level was significantly decreased by SIRT1-knockdown (si/shSIRT1) compared with control (si/shCon) in TOV21G cells. B and C: Western blotting showed the expression of CD44v and xCT, involved in the synthesis of GSH, and other antioxidative enzymes such as heme oxygenase-1 (HO-1) and thioredoxin (TRDX) in OvCa cell lines. Knockdown of SIRT1 by siRNA or shRNA (B) and EX527 (a SIRT1 inhibitor) (C) decreased expression of these antioxidative proteins. In contrast, overexpression of SIRT1 by SIRT1 cDNA increased expression of antioxidative proteins in ES2, and these effects of SIRT1 were canceled out by EX527 (C). Significance: * P < .05.