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. 2017 Jun 28;10(4):621–631. doi: 10.1016/j.tranon.2017.05.005

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Supplementary Figure S3 — A: Western blotting of SIRT1 and ACTB. The expression of SIRT1 protein in RMG1, A2780CDDP, TOV21G, and ES2 cells transfected with either shRNAs (shSIRT1–1 [shSIRT1], shSIRT1–2, and shSIRT1–3), siRNAs (siSIRT1-A, siSIRT1-B, and siSIRT1-C [siSIRT1]) sequences was suppressed compared with control cells transfected with the scrambled sequences (siCon and shCon). B: The effect of SIRT1 knockdown on cell proliferation was assessed using the WST-1 assay. Results were independently normalized by day 1. The knockdown of SIRT1 significantly decreased proliferation in OvCa cells (RMG1, A2780CDDP, TOV21G, and ES2). Results of shSIRT1–1 (shSIRT1) in RMG1 and A2780CDDP, and siSIRT1-C (siSIRT1) in TOV21G, and ES2 were shown in Fig. 2B. C: Western blotting of SIRT1 and ACTB. The expression of SIRT1 protein in RMG1, A2780CDDP, TOV21G, and ES2 cells transfected with SIRT1 cDNA (SIRT1) was enhanced compared with control cells transfected with empty vector (Con). Significance: * P < .05, significantly different from the controls.