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. 2017 Jun 29;7:4372. doi: 10.1038/s41598-017-04430-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transient expression of genes encoding necrosis and ethylene-inducing protein 1 (Nep1)-like protein (NLP) from Magnaporthe oryzae in Nicotiana benthamiana. MoNLP1, MoNLP2 and MoNLP4 trigger the accumulation of reactive oxygen species (A) and cell death (B), but not MoNLP3 and GFP control. Agrobacteria harboring individual MoNLP or GFP genes under the control of estrogen-inducible promoter were infiltrated into plant leaves. The estradiol solution (5 μM) was infiltrated into the inoculated area 24 hours post inoculation (hpi). Leaves were stained with DAB or trypan blue at 48 hpi.