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. 2017 Jun 29;7:4372. doi: 10.1038/s41598-017-04430-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic diagram of the vector pMFKO-DONR. f1 ori, phage f1 region; attP1 and attP2, Gateway sites; ccdB, coupled cell division B; Cm(R), chloramphenicol resistance gene; T_trpC, terminator of trpC gene; HSVtk, thymidine kinase of herpes simplex virus; P_ch, promoter region of Cochliobolus heterostrophus unknown protein presented in GenBank Accession Number M17304; T_35S, CaMV 3’UTR (polyA signal); hph, hygromycin B phosphotransferase gene; P_trpC, promoter region of trpC gene. ori, ColE1 origin of replication; Ap(R), ampicillin resistance gene.