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. 2017 Jun 29;7:4391. doi: 10.1038/s41598-017-04586-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro effects of G418 and gentamicin on selenoprotein biosynthesis. (A) HepG2 cells were incubated for 48 h in FCS-free medium with supplemental selenite (100 nM), G418 or gentamicin. Western blot analysis indicates increased secreted SELENOP protein levels in response to selenite and G418 treatment. Intracellular protein levels of selenoenzymes GPX1 and GPX2 were marginally affected. (B) The effect of gentamicin was verified in a dose-dependent analysis. (C) The effects of G418 on SELENOP secretion were replicated in human Hep3B, and (D) murine Hepa1-6 hepatocytes.