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. 2017 Jun 29;7:4391. doi: 10.1038/s41598-017-04586-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Se-content of SELENOP in relation to G418 and supplemental selenite. (A) The protein fraction of conditioned media from HepG2 cells was immobilized on a nitrocellulose membrane before and after immuno-precipitation of SELENOP (SEPP -IP). The SELENOP-IP procedure efficiently removed SELENOP (SEPP1), and with it all the detectable Se from the culture medium. (B) Supplemental selenite and G418 increased immuno-detectable SELENOP (SEPP1). (C) Se content of SELENOP preparations varied strongly between the different incubation conditions. (D) A comparison of the Se/SELENOP (Se/SEPP1) ratio indicates that G418 induces Sec-free SELENOP variants, whereas selenite increased the Se content of SELENOP in the presence of G418. (Mean ± SEM, n = 3).