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. 2017 Jun 29;7:4379. doi: 10.1038/s41598-017-04728-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GFP-D2 receptors show limited internalization. (A, C) Representative 2-photon images of live neurons at baseline, following 10 minutes of agonist bath application (quinpirole 10 µM-A, dopamine 100 µM-C), and following 30 minutes of agonist-free wash. Slices were incubated in anti-GFP-AF594 to label GFP-D2 receptors prior to imaging. (B) The change in fluorescence inside the neuron (ΔF/F) increases modestly but significantly over baseline following the agonist application and wash. There was no difference between the agonists used (n = 6 for both, two-way ANOVA followed by Bonferroni). (D) The change in the ratio of intracellular fluorescence to membrane fluorescence (ΔF_r/F_r) did not change significantly from baseline following agonist application or wash (n = 6 for both, two-way ANOVA followed by Bonferroni). *p < 0.05.