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. 2017 Jun 29;7:4363. doi: 10.1038/s41598-017-04718-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Three-dimensional spheroid culture using a PDMS-based hanging drop array (PDMS-HDA). (a) Fabrication of the PDMS-HDA device. (b) The left pictures show the fabricated PDMS-HDA device consisting of a 10*10 array of 1 μl drops dispensed by a liquid handing machine. The two micrographs on the right show the DI water drops with blue dyes on PDMS and polystyrene surfaces. (c) Procedure of 3D cell culture by the PDMS-HDA device. (d) Comparison of 3D MCF7 spheroids between the PDMS-HDA device and conventional HDA at different concentrations of collagen-I supplemented in medium at 24 h. The initial cell numbers per drop were 450. Scale bar, 500 μm; insert bar, 200 μm. (e) Comparison of MCF7 and MDA-MB-231 spheroids performed in 500 μg/ml and 1 mg/ml collagen-contained medium drops, respectively. Scale bar, 100 μm.