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. 2017 Jun 29;8:43. doi: 10.1038/s41467-017-00061-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — CIB2 deficiency leads to over-elongation of the transducing shorter row stereocilia in the auditory hair cells. a, b SEM images of OHC (a) and IHC (b) bundles from wild-type (left) and Cib2 ^tm1a/tm1a (right) littermate mice at P6 at the same cochlear location in the middle of the apical turn. Insets show magnified images of stereocilia tips and stereocilia links in the areas indicated by dashed boxes. c, d Quantification of the step height differences between the 1st (longest) and 2nd and between the 1st and 3rd rows of stereocilia (illustrated on the inset cartoons) in the OHC (c) and IHC (d) bundles at P6 (c, left and d) and P18 (c, right). Each dot represents one pair of stereocilia. Mean ± SEM values are also shown. We were not able to quantify the staircase morphology in the IHCs at P18 because mouse IHCs are very fragile at this age and their bundles lose cohesiveness after dissection. Number of cells: IHCs, wild-type, n = 6; Cib2 ^tm1a/tm1a, n = 6; OHCs at P6, wild-type, n = 9; Cib2 ^tm1a/tm1a, n = 18; OHCs at P18, wild-type, n = 10; Cib2 ^tm1a/tm1a, n = 10. In all panels, asterisks indicate statistical significance of the difference between the data from mutant and wild-type cells. NS, not significant; ****p < 0.0001 (ANOVA). Scale bars: 1 μm