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. 2017 Jun 29;8:43. doi: 10.1038/s41467-017-00061-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — CIB2 interacts with TMC1 and TMC2. a Fluorescent images of cells co-expressing LHFPL5-GFP, TMIE-GFP, CIB2-GFP (positive control), TMC1-EGFP or TMC2-EGFP (donor) and CIB2-V5 (acceptor) before and after acceptor photobleaching within the indicated region (white box). For negative control, we used TMC1-EGFP (donor) and CIB1-V5 (acceptor). FRET efficiency images are calculated as E _FRET = 100 × (I _Post−I _Pre)/I _Post, where I _Pre and I _Post are the EGFP pixel intensities before and after acceptor bleaching, respectively. Scale bars: 10 µm. b Quantitative analysis of FRET efficiency in CHO-K1 cells co-expressing LHFPL5-GFP, TMIE-GFP, CIB2-GFP, TMC1-EGFP or TMC2-EGFP (donor) and CIB2-V5 (acceptor). Quantitative analysis of negative control [TMC1-EGFP (donor) and CIB1-V5 (acceptor)] is also shown. Asterisks indicate statistical significance of FRET efficiency (p ≤ 0.0001). Error bars represent SEM. NS: not significant