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. 2017 Jun 29;7:4364. doi: 10.1038/s41598-017-04231-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effects of a P2X7R blockade on TJ distribution. Localization of occludin (A), claudin-1 (B), ZO-1 (C), and DAPI (DNA) within intestinal tissue sections assessed by immunofluorescence at 48 hours after CLP. TJ proteins (green) and DAPI stain (blue), merged TJ proteins and DAPI, as well as amplified merged TJ proteins and DAPI images are presented. Arrows indicate that biotin staining was ectopic to the lamina propria or deep within the epithelial surface. Arrowheads indicate the lack of focused staining on the surface of the villi.