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. 2017 Jun 30;8:731. doi: 10.3389/fimmu.2017.00731

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Copyright © 2017 Hearps, Agius, Zhou, Brunt, Chachage, Angelovich, Cameron, Giles, Price, Elliott and Jaworowski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD56^dim FcRγ⁻ NK cell expansion and increased human CMV (HCMV) antibodies are associated with MSM-related factors. (A) Plasma antibodies to the HCMV glycoprotein B (gB) were quantified by ELISA in plasma from HIV⁻ male controls recruited from either the community (n = 14) or from the Melbourne HIV Cohort consisting of men who have sex with men (MSM, n = 14). (B) The proportion of CD56^dimCD16⁺ NK cells lacking the FcRγ⁻ signal transduction protein was measured in peripheral blood mononuclear cells from the same individuals as in (A) using intracellular staining and flow cytometry. Graphs show Tukey plots of median (bar), IQR (box), and 1.5x IQR (whiskers); outliers are indicated by squares. p values shown were determined by Mann–Whitney U test. AU, Arbitrary units.