Figure 5.

T cell AICD and immune suppression induced by isoniazid can be overcome by curcumin nanoparticles. (A) Antigen-specific T cell proliferation. T lymphocytes were isolated from spleens of mice 40 days postinfection, and T cell proliferation assays were performed using tritiated thymidine. In vitro T cell proliferation of splenocytes from mice at different time points after infection was compared, after stimulation with Mycobacterium tuberculosis H37Rv complete soluble antigen (CSA). Data shown here are representative of five independent experiments with three mice in each group and represent the mean ± SD values. (B) Effect of isoniazid and curcumin nanoparticles on the activation of OT-II TCR transgenic T cells in the presence of ovalbumin (OVA) peptide. Splenocytes isolated from TCR Tg mice were cultured in the presence of 1 µg/mL OVA peptide along with 1 µg/mL INH and 10 µg/mL curcumin nanoparticles for 72 h. The cells were then stained and analyzed for T cell activation (CD44 and CD69) and cell death (7AAD) by FACS. The percentage of cells expressing CD44, CD69, or 7AAD among CD4⁺ T cells is shown with mean ± SD. Data shown here are representative of two independent experiments with two replicates in each group.