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. 2017 Jun 23;12:1811–1817. doi: 10.2147/COPD.S130616

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© 2017 Lacedonia et al. This work is published and licensed by Dove Medical Press Limited

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Evaluation of miRNA expression by qRT-PCR.

Notes: After normalization to miRNA-16 in each group, data were represented as mean ± SD and the obtained average values for each miRNA were used for statistics in ACOS, asthma, and COPD groups compared to healthy controls. Sputum miR-145 (A) was higher in asthma and COPD patients compared to healthy controls. At the same time, sputum miR-338 (B) was also present in higher abundance in all three groups analyzed, while there was no significant difference for serum miR-145 (C) in ACOS, asthma, and COPD patients compared to healthy controls. Serum miR-338 (D) was lower in ACOS, asthma, and COPD patients compared to healthy controls. The experiment was conducted in triplicate. *P<0.05.

Abbreviations: ACOS, asthma–COPD overlap syndrome; COPD, chronic obstructive pulmonary disease; qRT-PCR, quantitative real-time polymerase chain reaction.