Fig. 2.

Analysis of M1 markers in macrophages obtained from Elovl2^−/− and WT mice fed with diet enriched or not in DHA. Monocytes obtained from bone marrow of Elovl2^−/− (KO) and WT mice fed with diet enriched or not in DHA were differentiated into M1 macrophages using M-CSF (50 ng/ml) and IFN-γ (10 ng/ml) plus LPS (1 μg/ml). a mRNA analysis of iNOS and MARCO. Data are shown as mean (±sem) of ten independent experiments, each in duplicate. b CD80 and CD86 amount analyzed by flow cytometry upon staining cells at cell surface. Data are reported as mean fluorescence intensity (MFI), and are representative of eight independent experiments ± sem c ELISA of IL-6, IL-12p70 and IL-23 levels in supernatants of M1 macrophages. Data are reported as pg/ml, and are representative of five independent experiments ± sem, each in duplicate. d mRNA analysis of cyclooxygenases (COX-1 and COX-2) and lipoxygenases (5-LOX, 12-LOX, 15-LOX). *Denotes p < 0.05 versus WT; **Denotes p < 0.01 versus WT; #Denotes p < 0.05 versus KO; ^##denotes p < 0.01 versus KO