Fig. 2.

Inhibitory effect of γ-oryzanol on DNMTs in HFD-fed mice. HFD preference (a) and body weight (b) in γ-oryzanol-treated mice during food choice tests of chow vs HFD (n = 4 cages; three mice per cage). Levels of mRNA for Dnmt1 (c, g), Dnmt3a (d, h) and Dnmt3b (e, i) in the striatum (n = 8) (c–e) and hypothalamus (n = 8) (g–i) of γ-oryzanol (Orz)-treated HFD-fed mice. Levels were normalised to those of Rn18s. Protein levels of DNMT1 (k, l), DNMT3a (k, m) and DNMT3b (k, n) in the hypothalamus (Hypo) and striatum (St) of γ-oryzanol-treated HFD-fed mice (n = 8). Protein levels were determined by western blotting. The same membrane was used as in Fig. 5k. Values were normalised to those of β-actin. Activities of DNMTs (measured by SAH formation) in the striatum (n = 7) (f) and hypothalamus (n = 7) (j) of γ-oryzanol-treated HFD-fed mice. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01, vs chow-fed mice; ^† p < 0.05, ^†† p < 0.01 vs vehicle (Veh)-treated HFD-fed mice