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Physical map of the pAlgevir vector. The vector mediates the ethanol-inducible expression of the target protein through the AlcR transcription factor, which is constitutively expressed under the control of the Cauliflower mosaic virus 35S promoter and upon ethanol presence activates the AlcA promoter. The AlcA promoter and AlcR belong to Aspergillus nidulans. The Rep protein leads to replication of the expression cassette flanked by Ori elements.
