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. 2017 Jun 30;8:1151. doi: 10.3389/fpls.2017.01151

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Copyright © 2017 Kumar, Beena, Awana and Singh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative expression (RT-qPCR) analysis of salt-responsive high-affinity potassium transporter (HKT) genes. Transcript accumulation of TaHKT1;4 in root (A), TaHKT2;1 in shoot (B), TaHKT2;1 in root (C), TaHKT2;3 in shoot (D), and TaHKT2;3 in root (E). Two-week-old seedlings were treated with 200 mM NaCl for 14 days. KRL = KRL-210, KH = Kharchia-65, HD = HD-2329, and WH = WH-542. The results are mean fold change in relative expression over the control with three biological and three technical replicates, normalized with actin (reference) gene expression. Bars represent standard deviation. Columns with different lower case letters indicate significant difference at P < 0.05 (Fisher’s LSD test).