Figure 4.

Immunomagnetic (IM) sorting and immunofluorescence characterization of bone marrow (BM) micrometastatic cells in 'advanced disease' breast cancer patients. BM aspirates were immunopurified using MOC31-conjugated Dynabeads. Following IM sorting, immunofluorescence experiments were performed using anti-cytokeratin (a) and anti-CD45 (b) antibodies (green labeling), and were analyzed by light/fluorescence microscopy. Magnification, × 400 (a) and × 200 (b). These data are representative of experiments performed with BM aspirates from several patients.