Fig. 8.

Simulation of AVP responses in a model including ER Ca²⁺ dynamics. A: peaks per event as a function of variation in the 2 parameters g_BK and g_Nab. Three representative parameter combinations are indicated by markers for which AVP stimulation reproduces the experimental time courses. ER Ca²⁺ dynamics are included, and the calcium leak and inward rectifier conductances were increased to g_Ca,leak = 0.05 nS and g_Kir = 1.5 nS, respectively. Parameter combinations (g_Nab, g_BK): ◇, (0.14, 1); ○, (0.18, 0.85); □, (0.17, 0.35). All other parameter values had default values. B: time courses for AVP responses of the model at the 3 parameter combinations indicated by corresponding markers in A. AVP application is simulated beginning at time = 5 s by increasing the ER leak rate from default value p_rel = 0.0001 s⁻¹ transiently following a fast pulse profile (τ = 1 s, k_p = 10), and by simultaneously activating an inward ohmic conductance g_AVP following a slower pulse profile (τ = 12.5 s, parameter k_p = 1). To model the different doses of AVP in Fig. 2C, maximum increase in p_rel during the pulse was 0.01 s⁻¹, 0.0025 s⁻¹, and 0.001 s⁻¹, and peak g_AVP was 0.07 nS (top), 0.055 nS (middle), and 0.04 nS (bottom).