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NC canonical septin interfaces are not observed in CrSEPT crystals. A, superposing individual CrSEPT monomers across the NC interface of a homofilament composed of SEPT2. The arrow shows a minimal clash between the β-turns formed by strands β2 and β3 of each monomer. B, arrangement of CrSEPT dimers in crystal packing, showing how elements important for forming a canonical NC interface are unavailable because they are involved in packing contacts that stabilize the crystal.
