Figure 4.

EHF and FOXA1 regulate other transcription factors involved in airway epithelial differentiation in HBE cells. A, EHF depletion by siRNA followed by RT-qPCR identified changes in TF gene expression (n = 4). B, EHF ChIP-qPCR confirmed binding at specific sites identified in EHF ChIP-seq (n = 4). C, Genome Browser tracks show EHF peaks identified by IDR in HBE cells at the top (black), below are the EHF ChIP-seq tag counts (teal) and the gene (blue) for CEPBG (i), FOXA1 (ii), HOPX (iii), RARB (iv), and SPDEF (v). D, alterations in EHF levels and EHF-regulated TF gene expression after FOXA1 siRNA knockdown, shown by RT-qPCR (n = 3). E, FOXA1 ChIP qPCR confirmed its binding to a subset of EHF sites (n = 4). All experiments were performed in undifferentiated HBE cells. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; NS, not significant; unpaired two-tailed Student's t test. Bars show the average of all experiments, with error bars representing standard deviation.