Table 1.

Parameters of single-molecule motility assay of myosin VIIa dimer

Experiments were done on single actin filaments in the presence of 2 mMATP (see “Experimental Procedures”). Mean ± S.E. is shown unless otherwise indicated. ND, not determined.

Myosin Vlla	Step size	Dwell time, (τ)	Run length, (λ)	Velocity
	nm	s	μm	nm s−1
Drosophila myosin VIIaa	∼30	ND	ND	72 ± 20b
Human myosin VIIa (This study)	34.7 ± 11.5b	3.4 ± 0.2	0.71 ± 0.09	11.0 ± 0.6
	(n = 521)	(n = 387)	(n = 121)	(n = 86)

^a From Yang et al. (31).

^b Mean ± S.D.