Table 1.
ATPase assays
COS7 ATPase assays.
The results from ATPase assays presented in Fig. 6 for COS7 cells are summarized. The KCa2+ ([Ca2+] resulting in half-maximal activation) is given in pCa units (right-hand column) and nanomolar concentration (left-hand column). The change in KCa2+ (ΔKCa2+) relative to control (SERCA1 alone) is given in pCa units. Results are mean values ± S.E. NA means not applicable.
KCa2+ |
KCa2+ | n | ||
---|---|---|---|---|
nm [C] | pCa2+ | |||
SERCA1 alone | 468 ± 17 | 6.33 ± .016a | NA | 4 |
SERCA1 + sAnk1 | 708 ± 130 | 6.15 ± .089a,b,c | −0.18 | 4 |
SERCA1 + SLN | 1122 ± 190 | 5.95 ± .081a,b | −0.38 | 4 |
SERCA1 + sAnk1 + SLN | 759 ± 120 | 6.12 ± .071b,c | −0.21 | 4 |
a Data were previously shown in Ref. 1. All experiments presented were performed at same time.
b Data are significantly different from SERCA1 alone.
c Data are significantly different from SERCA1 + SLN.