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. 2017 May 9;292(26):10983–10997. doi: 10.1074/jbc.M117.780478

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Figure 3. — Spectra of reconstituted Rho-T4L compared with those of bovine Rho. A and B, UV-visible absorption spectra of Rho-T4L and Rho regenerated with 11-cis-retinal chromophore in the dark (black spectra) and after light illumination (gray spectra). C and D, UV-visible absorption spectra of Rho-T4L and Rho regenerated with 11-cis-6mr-retinal in the dark (red spectra) and after light illumination (gray spectra). E and F, chromophore release from purified Rho-T4L and bovine Rho reconstituted with 11-cis-retinal and 11-cis-6mr-retinal. Changes in the intrinsic Trp fluorescence were measured after a 15-s illumination through a 420–520-nm bandpass filter. E, chromophore release from Rho-T4L. F, chromophore release from Rho. Black spectra indicate samples regenerated with 11-cis-retinal, and red spectra indicate those regenerated with 11-cis-6mr-retinal.