Infected cells accumulate TAG LDs without OA treatment.
A–C, representative transmission electron micrographs of infected (A) versus uninfected (B) cells. The region noted in A is expanded in C, where apparently intravacuolar LDs are indicated by arrowheads. D, quantification of LD area normalized to total host cell area from 27 images for each condition. E, quantification of number of LDs per cell from same data set. In D and E, significance was calculated by Welch's unpaired t test. F, cumulative frequency distribution of individual LD areas from the quantification performed in E. Kolmogorov-Smirnov test: D = 0.10; ***, p < 0.0001.