Figure 8.

MYR1-mediated export is required for Toxoplasma-induced LD accumulation. A, representative fluorescence micrographs of BODIPY-stained neutral lipids of HFFs infected for 24 h after infection with the indicated strain. DIC, differential interference contrast. B, TAG quantification of cells treated with OA and infected with the indicated strain for 24 h (n = 3). C, qPCR quantification of host transcripts of cells infected for 24 h with the indicated strain without the addition of OA. D, Western blots of lysates from cells infected with wild-type RH or RHΔmyr1 parasites or left uninfected (Uninf) and probed with the indicated antibodies.