Figure 3.

SirE up-regulates production of extracellular hydrolases. A. nidulans A26 (WT) (●) and SirEΔ (■) were cultured in liquid GMM at 30 °C, and then hydrolase activities in culture supernatants were determined. A, B, and C, chitinase, β-1,3-glucanase, and N-acetyl-d-glucosaminidase, respectively. Data are means ± S.E. of three experiments. *, p < 0.05 versus WT. D, amounts of gene transcripts in 48-h cultures of SirEΔ determine by real-time PCR. Values are relative to those of WT. Data are means ± S.E. of three experiments. Error bars represent S.E. p < 0.05.