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. 2017 Jun 30;8:1120. doi: 10.3389/fpls.2017.01120

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Copyright © 2017 Kumar, Gho, Jung and Kim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of expression patterns for two cold stress-responsive genes using GUS reporter systems. Promoter trap lines using GUS reporter gene were selected and tested for GUS activity. Promoter trap line for LOC_Os01g31370, Line PFG 3A-50649 (right), and that of LOC_Os03g49830, Line PFG 1C-08613 (left) were confirmed through co-segregation test of GUS expression and T-DNA insertion through genotyping analysis. Upper panel, GUS-staining data from promoter trap lines under normal growing conditions; lower panel, lines under cold-stress conditions. Homozygous progeny of T-DNA insertion for each of two lines were used.