Figure 5. Skeletal muscle ROS production is increased in rats with ‘ligated’ femoral arteries.

A, representative 20× images of pseudo‐coloured, red ethidium‐positive nuclei in plantaris muscle cross‐sections (scale bar = 100 μm; n = 2). The number of ethidium‐positive nuclei was increased in muscle from ‘ligated’ rats (middle) relative to ‘freely perfused’ controls (top) and was reduced following intra‐arterial tiron infusion (bottom). B, similarly, in vitro ethidium fluorescence in the presence of the Nox substrate, NADH, was quantitatively augmented in ‘ligated’ (n = 6) vs. ‘freely perfused’ rats (n = 6) and reduced with tiron administration in both groups, although more so in ‘ligated’ animals. C, lucigenin‐derived chemiluminescence in the presence of NADH was increased in ‘ligated’ (n = 8) vs. ‘freely perfused’ rats (n = 8) and was reduced similarly in both groups by tiron. ^* P < 0.05, ^** P < 0.01 and ^*** P < 0.001 for bracketed comparison. [Color figure can be viewed at wileyonlinelibrary.com]