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. 2017 Jun 30;7:300. doi: 10.3389/fcimb.2017.00300

Figure 1.

Figure 1

SlaA stimulated the adhesion of THP-1 cells to HUVECs. (A) HUVECs were incubated with various concentrations (A) or 1 μg/ml (B) of SlaA or C134A at 37°C for 6 h. The cells were incubated with CFSE-labeled THP-1 cells at 37°C for 24 h (A) or the indicated times (B). These cells were washed and the labeled THP-1 cells were viewed with a fluorescent microscope. The fluorescent intensity of CFSE-labeled THP-1 cells was quantified as described in Materials and Methods. (C,D) HUVECs (C) and THP-1 cells (D) were incubated with various concentrations of SlaA and C134A at 37°C for 24 h, and then stained with AlamarBlue. The results shown represent the mean ± SEM; n = 4.