Skip to main content
. 2017 Jun 30;7:300. doi: 10.3389/fcimb.2017.00300

Figure 2.

Figure 2

Streptococcous pyogenes culture supernatant induces the adhesion of THP-1 cells to HUVECs. (A) Protein expression of SlaA and GAPDH/Plr from S. pyogenes wild-type strain SSI-I and its ΔSlaA isogenic mutant strain was detected by Western blotting. (B) HUVECs were pre-incubated with THY broth, S. pyogenes SSI-I culture supernatant, or its ΔSlaA mutant strain culture supernatant at 37°C for 6 h. The cells were incubated with CFSE-labeled THP-1 cells at 37°C for 24 h. These cells were washed and the CFSE-labeled THP-1 cells were viewed with a fluorescent microscope. The fluorescent intensity of CFSE-labeled cells was quantified as described in Materials and Methods. The results shown represent the mean ± SEM; n = 5. Data were analyzed using a one-way ANOVA with Dunnett's multiple comparison test. Significant differences from the S. pyogenes SSI-I culture supernatant group are shown: *P < 0.01.