Figure 3.

SlaA induced the expression of adhesion molecules in HUVECs. HUVECs were incubated with 1 μg/ml SlaA or C134A at 37°C for 3 h (A–D) and 6 h (E–H). The transcription of ICAM1 (A), VCAM1 (B), E-selectin (C), and P-selectin (D) mRNAs in HUVECs was measured by real-time PCR, as described in Materials and Methods. The relative quantity of these mRNAs was normalized to the relative quantity of GAPDH mRNA. The protein expression of ICAM1 (E), VCAM1 (F), E-selectin (G), and P-selectin (H) in HUVECs was analyzed by immunostaining using fluorescently labeled antibodies. The fluorescence intensity of each protein was quantified as described in Materials and Methods. The results shown represent the mean ± SEM; n = 5. Data were analyzed using one-way ANOVA with Dunnett's multiple comparison test. Significant differences from the PBS group are shown: ^*P < 0.01.