Figure 1.
Treatment protocols. (A) Mice were sensitized at days 0 and 7 and subjected to a series of four provocations with ovalbumin (OVA) (50 μg/25 μL) at days 14, 21, 28, and 35 post-sensitization. 15d-PGJ2 (30 or 100 μg/kg) was subcutaneously injected at days 28 and 35, 30 min before OVA; (B) mice were challenged with house dust mite extract (HDM) (15 μg/15 μL/mouse) intranasally, three times per week during 3 weeks. Treatments with 15d-PGJ2 occurred at the last week, as administered subcutaneously (30–100 μg/kg) or intranasally (0.7–2.3 μg/25 μL/mouse), 30 min before provocation.