Figure 11.

15d-PGJ₂ inhibits mast cell degranulation in vitro. RBL-2H3 cells (1.25 × 10⁵ cells/well) were sensitized with IgE anti-DNP (1 µg/ml), incubated for 20 h, and treated with 15d-PGJ₂ (5–20 µM) (A), pretreated or not with GW662 (0.1–10 µM) (B), and stimulated with DNP–BSA (10 ng/ml) 45 min later. The β-hexosaminidase levels were quantified 45 min after stimulus using a spectrophotometer at 405 nm. These results are expressed as the mean ± SEM from four independents experiments. ⁺P < 0.05 as compared to unchallenged group; *P < 0.05 as compared to DNP–BSA challenged group.