Figure 2.

15d-PGJ₂ reduces peribronchiolar infiltration of eosinophils and neutrophils induced by ovalbumin (OVA) challenge. Photomicrographs of representative airways in Sirius Red-stained lung sections from mice challenged with saline (A), OVA (B), and OVA + 15d-PGJ₂ (100 μg/kg, s.c.) (C). White, yellow, and red arrows indicate mononuclear cell, eosinophil, and neutrophil, respectively. The number of mononuclear cells (D), eosinophils (E), and neutrophils (F) in peribronchiolar regions were counted in 7–10 bronchioles per mouse. Treatments were carried out 30 min before OVA exposure during the last 2 weeks of provocation. All samples for histologic examinations were undertaken 24 h after the last OVA challenge. Each value represents the mean ± SEM from seven animals. Br, bronchiolar lumen. Scale bar = 40 µm. ⁺P < 0.05 as compared to saline-challenged group; *P < 0.05 as compared to OVA-challenged group.