Figure 2. Effects of SBRE on H₂O₂-induced ROS generation in HaCaT keratinocyte cells. Cells were pretreated with 600 μg/ml SBRE or 10 mM NAC for 1 h and then stimulated with or without 1 mM H₂O₂ for 30 min. The cells were incubated at 37 °C in the dark for 20 min with culture medium containing 10 μM DCF-DA to monitor ROS production. (A) ROS generation was measured by flow cytometry. (B) Each point represents the mean ± SD of three independent experiments (*P < 0.05 vs. untreated control; ^#P < 0.05 vs. H₂O₂-treated cells).