Figure 2.

Four out of six anti-CD100 monoclonal antibody clones inhibit T cell proliferation. (A) Staining of activated peripheral blood mononuclear cells (PBMCs) with isotype control purified (gray) or different clones of anti-human CD100 purified (red) followed by anti-mouse IgG or IgM APC. PBMCs were stimulated by anti-CD3/CD28. CD3⁺ T cells were used for analysis. Experiments were repeated twice with two different PBMC preparations. (B) Total PBMCs pre-stained with Carboxyfluorescein 6 succinimidyl ester (CFSE) and cultured with Dynabeads anti-CD3/CD28 in the presence of indicated soluble anti-CD100 clones, respectively (10 µg/ml). Representative histograms of CFSE fluorescence on T cells after 5 days of culture were shown. Isotype indicates mouse IgM with azide. (C) Summary of data showing the inhibition ratio of each clone (n = 2–6). Experiments were repeated three times with six PBMC preparations in total.